Journal: PLoS ONE

Article Title: Evaluation of the Protective Immunity of a Novel Subunit Fusion Vaccine in a Murine Model of Systemic MRSA Infection

doi: 10.1371/journal.pone.0081212

Figure Lengend Snippet: BALB/c mice were immunized with recombinant proteins as described in the Materials and Methods. One week after the last booster, the mice (n = 6) were bled, and the sera were tested by ELISA for anti-recombinant protein antibodies. The titers were determined for IgG (A), IgG1 (B), and IgG2a (C). The results represent the means and standard error for a group of mice.

Article Snippet: The amounts of IFN-γ, IL-5, and IL-17A in the supernatants were determined by ELISAs using a mouse IFN-γ valukineTM ELISA kit, mouse IL-5 valukineTM ELISA kit, and IL-17A valukineTM ELISA kit (R&D Systems), respectively.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Evaluation of the Protective Immunity of a Novel Subunit Fusion Vaccine in a Murine Model of Systemic MRSA Infection

doi: 10.1371/journal.pone.0081212

Figure Lengend Snippet: BALB/c mice were immunized with individual recombinant proteins or injected with antigen-free PBS and alum adjuvant. Two weeks after the last booster, the mice were infected with S. aureus MRSA252 (2.5×10 8 CFU). Three days after infection, the kidneys of the mice were obtained and homogenized. The cytokine levels in organ homogenates were determined by ELISA. (A)IFN-γ, (B) IL-17A, and (C) CXCL2 expression in the kidneys. Each group included 5 mice, and the medians and interquartile ranges are shown. * P <0.05, ** P <0.01 vs. control by the Mann-Whitney U test.

Article Snippet: The amounts of IFN-γ, IL-5, and IL-17A in the supernatants were determined by ELISAs using a mouse IFN-γ valukineTM ELISA kit, mouse IL-5 valukineTM ELISA kit, and IL-17A valukineTM ELISA kit (R&D Systems), respectively.

Techniques: Recombinant, Injection, Adjuvant, Infection, Enzyme-linked Immunosorbent Assay, Expressing, Control, MANN-WHITNEY